Application note: Direct PCR from blood using Platinum SuperFi DNA Polymerase

This application note describes how researchers can successfully perform PCR from blood samples without DNA purification using Platinum SuperFi DNA Polymerase…

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors present in blood, as well as to most widely used blood preservatives (e.g., heparin, EDTA, citrate).

This application note describes how researchers can successfully perform PCR from blood samples without DNA purification using Platinum SuperFi DNA Polymerase. Multiplex PCR can also be used to amplify more than one target sequence directly from blood. This protocol minimizes sample loss and helps significantly reduce protocol time with no DNA purification or isolation steps.

Materials and methods

• Whole blood
• Invitrogen™ Platinum™ SuperFi™ PCR Master Mix (Cat. No. 12358010) or Platinum SuperFi DNA Polymerase (Cat. No. 12351010)
• Invitrogen™ dNTP Mix (10 mM each, Cat. No. 18427013; only required for stand-alone enzyme)
• Forward and reverse primers
• 50 mM MgCl2
• Invitrogen™ E-Gel™ General Purpose Agarose Gels or equivalent