Application note: Middle-up mass analysis of protease digested cetuximab

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Posted: 30 November 2022 | Merck | No comments yet

Protocol for purification, proteolysis, reduction and UHPLC-MS analysis of unlabelled and labelled monoclonal antibodies

This application note details a complete workflow of a middle-up mass analysis of protease digested Cetuximab monoclonal antibody, starting with the antibody purification, proteolysis and reduction to its reversed phase UHPLC-MS analysis.

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