Application note: Workflow for high-throughput glycoprofiling of rituximab
Posted: 1 November 2022 | Merck | No comments yet
Protocol for purification, reduction, and SEC-MS glycoform analysis of a therapeutic monoclonal antibody (mAb)
Discover in this application note the complete SEC-MS workflow, developed to enable rapid glycoprofiling of monoclonal antibodies (mAbs) in cell culture supernatants. It includes:
- Antibody (mAb) purification using immobilised protein A
- Antibody reduction procedure
- Mass spectrometer calibration
- System suitability test utilising a recombinant human monoclonal antibody reference
- Size exclusion chromatography – mass spectrometry (SEC-MS) method for sample separation and analysis.
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