Application note: Workflow for high-throughput glycoprofiling of rituximab

Protocol for purification, reduction, and SEC-MS glycoform analysis of a therapeutic monoclonal antibody (mAb)

Discover in this application note the complete SEC-MS workflow, developed to enable rapid glycoprofiling of monoclonal antibodies (mAbs) in cell culture supernatants. It includes:

• Antibody (mAb) purification using immobilised protein A
• Antibody reduction procedure
• Mass spectrometer calibration
• System suitability test utilising a recombinant human monoclonal antibody reference
• Size exclusion chromatography – mass spectrometry (SEC-MS) method for sample separation and analysis.

Read the application note