Application note: Analysis of voglibose by postcolumn derivatisation method
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Published: 26 October 2022
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This application note introduces the analysis of voglibose tablets as described in the 17th Edition of Japanese Pharmacopoeia.
Quality control of voglibose by high-performance liquid chromatography (HPLC) can be challenging, as there is no chromophore or conjugation in the molecule. Moreover, it does not exhibit ultraviolet (UV) absorption, which makes it difficult to detect.
Monographs for purity test and assay of voglibose tablets described in the Japanese Pharmacopoeia (JP) define an HPLC method for analysis with post-column derivatisation prior to fluorescence detection.
This application note describes a simple and reliable instrument setup for the analysis of the alpha glucosidase inhibitor voglibose. Using a dedicated chemical reaction box for the post-column derivatisation procedure, combined with a standard HPLC system with fluorescence detector, the assay meets all performance goals with regard to repeatability, robustness and sensitivity.
Download this free application note to learn more…
