Whitepaper: Innovative analytical techniques for bioprocess investigation

WITH THE RISE of metabolites/molecules production using complete living cells or their components (bacteria, enzymes, chloroplasts, etc), which is also termed bioprocessing, it has become increasingly important to control the different production steps and analyse the different biological materials.

Some techniques, such as fluorescence and Raman spectroscopy, dynamic light scattering or nano tracking analysis and surface plasmon resonance imaging (SPRi) can be very useful in the characterisation of samples.

On one hand, spectroscopic-based systems such as fluorescence and Raman can be applied at the stage of upstream processes for cell culture analysis. Fluorescence spectroscopy has shown to be a rapid method of identifying and evaluating the quality of cell culture media. Using Raman spectroscopy combined with microscopy and multivariate analysis, different species and growth phases of single bacteria cells can be quickly distinguished.