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HPLC

 

Universal detection in high performance liquid chromatography
Universal detection in high performance liquid chromatography
article

Universal detection in high performance liquid chromatography

3 July 2015 | By David P. Elder, Phil Borman and George Okafo - GlaxoSmithKline

The most commonly used analytical technique for the testing of drug molecules is reversed-phase high performance liquid chromatography (RP-HPLC) with ultraviolet (UV)/visible detection. However, one of the biggest constraints affecting UV/visible detectors is their inability to generate adequate sensitivity for analytes lacking a suitable UV chromophore.

LC-MS In-Depth Focus 2015
LC-MS In-Depth Focus 2015
article

LC-MS In-Depth Focus 2015

20 April 2015 | By

In this free-to-view LC-MS in-depth focus, we delve into the world of glycans, and how their natural complexity necessitates sophisticated sequencing techniques, while another article aims to demonstrate the versatility of LC-MS and the ease with which it can be combined with a variety of MS systems, focusing particularly on…

HPLC: On-chip liquid chromatographic separation
HPLC: On-chip liquid chromatographic separation
article

HPLC: On-chip liquid chromatographic separation

17 April 2015 | By

High-performance liquid chromatography (HPLC) is a powerful separation technique due to its high accuracy, precision, versatility and robustness. In the development of pharmaceuticals, it is no doubt that LC is a key technology in the quality control of pharmaceuticals and pharmacokinetic studies and in the analysis of complex biological compounds…

David Elder
David Elder
article

Can HPLC assay methods really be considered quality critical tests?

5 September 2014 | By

Analytical method specificity is assessed using ICH (International Conference on Harmonisation) Q2 (2005). Although, certain methods are not specific enough for their intended purposes, they may have other advantages. Both titrimetric and UV (Ultra-violet spectroscopy) assays are non-specific, but have superior precision (ca. 0.1-0.5% RSD (Residual Standard Deviation)) compared with…