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Jim Huggett

qPCR: Current technologies and future applications

A diverse and widely applicable laboratory technique, qPCR is vital…

9 May 2010 | By European Pharmaceutical Review

A diverse and widely applicable laboratory technique, qPCR is vital for the progression of drug discovery, enabling detection and quantification and commonly used for both diagnostic and basic research. This roundtable brings together experts from a wide range of pharmaceutical applications to discuss current technologies and future applications of qPCR…

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Digital polymerase chain reaction; new diagnostic opportunities

LGC is an international science-based company located in South West…

22 February 2010 | By

LGC is an international science-based company located in South West London. A progressive and innovative enterprise, LGC operates in socially responsible fields underpinning the health, safety and security of the public, and regulation and enforcement for UK government departments and blue chip clients. Our products and services enable our customers…

article

Article 5: qPCR data analysis – Amplification plots, Cq and normalisation

A pivotal attraction of qPCR technology is its apparent lack…

9 October 2009 | By

A pivotal attraction of qPCR technology is its apparent lack of complication; an assay consisting of the simple procedure of combining oligonucleotides, PCR mastermix buffer and nucleic acid template to produce a qPCR reaction is perceived as undemanding. This practical simplicity is complemented by the absence of any requirement for…

article

There is more to qPCR than the PCR reaction

The polymerase chain reaction is arguably the most significant technical…

29 September 2008 | By

The polymerase chain reaction is arguably the most significant technical discovery yet to have been made in the field of molecular biology and genetics, if not all life science. It cannot be overstated how much of an impact this technique has had, resulting in molecular biology becoming an integral part…

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Recommended

Jim Huggett

qPCR: Current technologies and future applications

Digital polymerase chain reaction; new diagnostic opportunities

Article 5: qPCR data analysis – Amplification plots, Cq and normalisation

There is more to qPCR than the PCR reaction