Application Note: Evolving promising leads

The precision of data from surface plasmon resonance (SPR)-based analyses, and the confidence with which results can be interpreted, is strongly coupled to the sensitivity of the SPR sensor. Biacore™ T200 system from GE Healthcare offers excellent sensitivity that enables detection of the lowest molecular-weight interactions and accurate measurements at very low concentrations.

The system allows generation of high-quality data even when working with unstable and sensitive molecules such as membrane proteins.

Advantages of kinetics in screening and characterisation Interactions characterised by similar affinities can have very different kinetics. By resolving affinity into on- and off-rates, comprehensive information is obtained on how the dynamics of molecular interactions relate to protein function. On-rates reflect recognition between interacting partners, while off-rates indicate the stability of the complex. This information provides an extra dimension which can be crucial in supporting hit-to-lead development…