Application Note: Anthocyanin purification from blackcurrant with SCPC-100
Posted: 9 January 2015 | Gilson Europe
The blackcurrant is the edible berry of a shrub, which usually grows to 1-2 meters and can be found in Northern/Central Europe and Asia.
Anthocyanins are found in high concentrations in the fruit and give its characteristic dark purple color. Delphinidin 3-O-glucoside, delphinidin 3-Orutoside, cyanidin 3-O-glucoside and cyanidin 3-0-rutoside make up more than 97% of anthocyanin in the plant1. The crude extract is a complex matrix and the use of Centrifugal Partition Chromatography (CPC) enables purification of the targeted compound. 500mg of powder extract of blackcurrant was injected by CPC to purify anthocyanin.
Apparatus
An Armen SCPC-100 with 100mL column capacity was connected and controlled via an Armen Spot prep II system equipped with 50ml/mn quaternary gradient pump, UV/Vis detector and fraction collector. AGCPC software was used…
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