Application note

Application note: Workflow for antibody aggregate and fragment analysis

Posted: 14 December 2022 | | No comments yet

Protocol for heat stressing and SEC-UV analysis of monoclonal antibody aggregates and fragments.

A complete SEC-UV workflow has been developed to enable quantitative analysis of antibody fragments and aggregates. In detail, it includes:

  • Procedure for heat stressing of antibodies
  • System suitability test utilising a gel filtration standard
  • SEC-UV method for sample separation and analysis.

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