Whitepaper: A comparison of the attributes of the AccuGENX-ID microbial sequencing methods versus MicroSEQ v2.0
Posted: 2 March 2012 | Accugenix | No comments yet
This whitepaper offers a detailed analysis of the differences between AccuGENX-ID™ and the MicroSEQ® v2.0* methodologies in obtaining an accurate identification.
We focus on 4 areas that impact the accuracy of microbial identifications: 1) Library relevance, depth of coverage and validation; 2) Analyzing the quality of the data generated; 3) Data assembly analysis and distance measurements and 4) Results Interpretation. The semi-automated methods used by Accugenix can be traced back to the reference methods used by taxonomists, and these methods analyze microbial sequence data through a conventional assembly process. This process includes autoassembly, manual review, correction of base caller errors and demonstrates a 98% accuracy and six-fold better repeatability than the fully automated method used by MicroSEQ v2.0.
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